BIO CHEMISTRY MCQs (PART 1)

1. In severe acidosis, the output of urea is

(A) Decreased

(B) Slightly increased

(D) Moderately increased

2. Uremia occurs in

(A) Cirrhosis of the liver

(B) Nephritis

(D) Coronary thrombosis

3. Clinical symptom in urea cycle disorder is

(A) Mental retardation

(B) Drowsiness

(D) Oedema

4. The sparing action of methionine is

(A) Tyrosine

(B) Cystine

(D) Tryptophan

5. NH+ 4 aminates glutamate to form glutamine requiring ATP and

(A) K+

(B) Na+

(D) Mg++

6. Glutathione is a

(A) Dipeptide

(B) Tripeptide

(D) None of these

7. All following are conjugated proteins except

(A) Nucleoproteins

(B) Proteoses

(D) Flavoproteins

8. All α-amino acids have one asymmetric carbon atom except

(A) Arginine

(B) Glycine

(D) Histidine

9. Number of amino acids present in plants, animals and microbial proteins:

(A) 20

(B) 80

(D) 200

10. Hydrated density of (HD) lipoproteins is

(A) 0.94 gm/ml

(B) 0.94-1.006 gm/ml

(D) 1.063-1.21 gm/l

11. The bond in proteins that is not broken under usual conditions of denaturation:

(A) Hydrophobic bond

(B) Hydrogen bond

(D) Peptide bonds

12. Plasma proteins act as

(A) Buffers

(B) Immunoglobulins

(D) All of these

13. Group that reacts in the Biuret test:

(A) Peptide

(B) Amino group

(D) Aldehyde group

14. In nitroprusside test, amino acid cysteine produces a:

(A) Red colour

(B) Blue colour

(D) Purple colour

15. Protein present in hemoglobin has the structure known as

(A) Primary

(B) Secondary

(D) Quarternary

16. Isoelectric pH of an amino acid is that pH at which it has a

(A) Positive charge

(B) Negative charge

(D) None of these

17. Albuminoids are similar to

(A) Albumin

(B) Globulin

(D) None of these

18. Optical isomers of all aminoacids exist except

(A) Glycine

(B) Arginine

(D) Hydroxy proline

19. Proteins that constitute keratin, collagen and elastin in body are

(A) Protamines

(B) Phosphol proteins

(D) Metaproteins

20. Systematic name of lysine is

(A) Amino acetic acid

(B) 2,6 diaminohexanoic acid

(D) 2-Aminopropanoic acid

21. Side chains of all following amino acids contain aromatic rings except

(A) Phenyl alanine

(B) Alanine

(D) Tryptophan

22. Abnormal chain of amino acids in sickle cell anaemia is

(A) Alpha chain

(B) Beta chain

(D) Gama chain

23. Number of chains in globin part of normal Hb:

(A) 1

(B) 2

(D) 4

24. The PH of albumin is

(A) 3.6

(B) 4.7

(D) 6.1

25. Ninhydrin reaction gives a purple colour and evolves CO2 with

(A) Peptide bonds

(B) Histamine

(D) Aspargine

26. Denaturation of proteins involves breakdown of

(A) Secondary structure

(B) Tertiary structure

(D) All of these

27. In denaturation of proteins, the bond which is not broken:

(A) Disulphide bond

(B) Peptide bond

(D) Ionic bond

28. The purity of an isolated protein can be tested by employing various methods.

(A) Solubility curve

(B) Molecular weight

(D) Immuno Ractivity

(E) All of these

29. More than one break in the line or in saturation curve indicates the following quality of protein.

(A) Non homogenity

(B) Purity

(D) None of these

30. A sharp moving boundary is obtained between the pure solvent and solute containing layer in

(A) Chromatography

(B) Immuno Reactivity

(D) Solubility curve

31. The antibodies raised against a pure protein will show only one sharp spike on this technique:

(A) Solubility curve

(B) Solvent precipitation

(D) Immuno electrophoresis

32. This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH:

(A) Isoelectric focussing

(B) Immunoel Ectro Phoresis

(D) HPLC

33. The following technique makes use of the difference in net charges of proteins at a given pH:

(A) Thin layer chromatography

(B) Ion exchange chromatography

(D) Paper chromatography

34. The ratio of the distance moved by a compound to the distance moved by the solvent frent is known as its

(A) PI value

(B) Linking number

(D) Gold number

35. The movement of charged particles towards one of the electrodes under the influence of electrical current is

(A) Gel filtration

(B) Molecular sieving

(D) Electrophoresis

36. An anion exchange resin linked to cellulose backbone is

(A) DEAE cellulose

(B) CM cellulose

(D) None of these

37. A cation exchange resin linked to cellulose backbone is

(A) CM-cellulose

(B) DEAE cellulose

(D) Biogel

38. The sorting out of molecules according to size and shape may be adapted to protein purification in this technique:

(A) Adsorption chromatography

(B) Gel filtration chromatography

(D) None of these

39. Frequently employed materials for the adsorption chromatography of proteins include

(A) High capacity supporting gel

(B) Starch blocks

(D) All of these

40. The solubility of most proteins is lowered at high salt concentrations is called as

(A) Salting in process

(B) Salting out process

(D) None of these

41. Phenylalanine, ornithine and methionine are involved in the biogenesis of

(A) Lysergic acid

(B) Reserpine

(D) Papaverine

42. All the following diuretics inhibit the carbonic anhydrase except

(A) Acetazolamide

(B) Bumetanide

(D) Ethacrynic acid

43. Protein is a polymer of

(A) Sugars

(B) Phenols

(D) Carboxylic acids

44. All the following amino acids are optically active except

(A) Tryptophane

(B) Phenylalanine

(D) Glycine

45. Proteinous substances which catalyze biochemical reactions are known as

(A) Activators

(B) Catalysts

(D) Hormones

46. Insulin is a protein which controls

(A) Blood clotting

(B) Metabolic pathway

(D) Kreb’s cycle

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BIO CHEMISTRY MCQs (PART 1)