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Free Bio Chemistry MCQs with Answer

Free Bio Chemistry MCQs with Answer






1. At a pH below the isoelectric point, an amino acid exists as
(A) Cation
(B) Anion
(C) Zwitterion
(D) Undissociated molecule

2. An amino acid having a hydrophilic side chain is
(A) Alanine
(B) Proline
(C) Methionine
(D) Serine

3. An amino acid that does not take part in α helix formation is
(A) Histidine
(B) Tyrosine
(C) Proline
(D) Tryptophan

 4. A protein rich in cysteine is
(A) Collagen
(B) Keratin
(C) Haemoglobin
(D) Gelatin

5. Primary structure of proteins can be determined by the use of
(A) Electrophoresis
(B) Chromatography
(C) Ninhydrin
(D) Sanger’s reagent

6. Electrostatic bonds can be formed between the side chains of
(A) Alanine and leucine
(B) Leucine and valine
(C) Asparate and glutamate
(D) Lysine and aspartate

7. Sanger’s reagent contains
(A) Phenylisothiocyanate
(B) Dansyl chloride
(C) 1-Fluoro-2, 4-dinitrobenzene
(D) Ninhydrin

8. The most abundant protein in mammals is
(A) Albumin
(B) Haemoglobin
(C) Collagen
(D) Elastin

9. Folding of newly synthesized proteins is accelerated by
(A) Protein disulphide isomerase
(B) Prolyl cis-trans isomerase
(C) Chaperonins
(D) All of these

10. Primary structure of a protein is formed by
(A) Hydrogen bonds
(B) Peptide bonds
(C) Disulphide bonds
(D) All of these

11. α-Helix is formed by
(A) Hydrogen bonds
(B) Hydrophobic bonds
(C) Electrostatic bonds
(D) Disulphide bonds

12. Glutelins are present in
(A) Milk
(B) Eggs
(C) Meat
(D) Cereals

13. Aromatic amino acids can be detected by
(A) Sakaguchi reaction
(B) Millon-Nasse reaction
(C) Hopkins-Cole reaction
(D) Xanthoproteic reaction

14. Two amino groups are present in
(A) Leucine
(B) Glutamate
(C) Lysine
(D) Threonine

15. During denaturation of proteins, all of the following are disrupted except
(A) Primary structure
(B) Secondary structure
(C) Tertiary structure
(D) Quaternary structure

 16. All the following are branched chain amino acids except
(A) Isoleucine
(B) Alanine
(C) Leucine
(D) Valine

17. An –OH group is present in the side chain of
(A) Serine
(B) Arginine
(C) Lysine
(D) Proline


18. Edman’s reagent contains
(A) Phenylisothiocyanate
(B) 1-Fluoro-2, 4-dinitrobenzene
(C) Dansyl Chloride
(D) tBOC azide

19. Edman’s reaction can be used to
(A) Determine the number of tyrosine residues in a protein
(B) Determine the number of aromatic amino acid residues in a protein
(C) Determine the amino acid sequence of a protein
(D) Hydrolyse the peptide bonds in a protein

20. Inherited deficiency of β−glucosidase causes
(A) Tay-Sachs disease
(B) Metachromatic leukodystrophy
(C) Gaucher’s disease
(D) Multiple sclerosis

21. Tay-Sachs disease results from inherited deficiency of
(A) Arylsulphatase A
(B) Hexosaminidase A
(C) Sphingomyelinase
(D) Ceramidase

22. The largest alpolipoprotein is
(A) Apo E
(B) Apo B-48
(C) Apo B-100
(D) Apo A-I

23. Apolipoprotein B-100 is synthesised in
(A) Adipose tissue
(B) Liver
(C) Intestine
(D) Liver and intestine

24. Apolipoprotein B-48 is synthesized in
(A) Adipose tissue
(B) Liver
(C) Intestine
(D) Liver and intestine

 25. Apolipoproteins A-I and A-II are present in
(A) LDL only
(B) LDL and VLDL
(C) HDL only
(D) HDL and chylomicrons

 26. Apolipoprotein B-48 is present in
(A) Chylomicrons
(B) VLDL
(C) LDL
(D) HDL

27. Apolipoprotein B-100 is present in
(A) Chylomicrons
(B) VLDL only
(C) LDL only
(D) VLDL and LDL

28. Apolipoproteins C-I, C-II and C-III are present in
(A) Chylomicrons
(B) VLDL
(C) HDL
(D) All of these

29. Apolipoprotiens C-I, C-II and C-III are present in all of the following except
(A) Chylomicrons
(B) VLDL
(C) LDL
(D) HDL

 30. Apolipoprotein A-I acts as
(A) Enzyme activator
(B) Ligand for receptor
(C) Both (A) and (B)
(D) None of these

31. Apolipoprotien B-100 acts as
(A) Enzyme activator
(B) Ligand for receptor
(C) Both (A) and (B)
(D) None of these

 32. Apolipoprotein C-II is an activator of
(A) Lecithin cholesterola acyl transferase
(B) Phospholipase C
(C) Extrahepatic lipoprotein lipase
(D) Hepatic lipoprotein lipase

33. Nascent chylomicron receives apolipoproteins C and E from
(A) VLDL remnant
(B) VLDL
(C) LDL
(D) HDL

34. Terminal transferase
(A) Removes nucleotides from 3’ end
(B) Adds nucleotides at 3’ end
(C) Removes nucleotides from 3’end
(D) Adds nucleotides at 3’end

35. S1 nuclease hydrolyses
(A) DNA of somatic cells
(B) DNA of sperms
(C) Any double stranded DNA
(D) Any single stranded DNA

36. Positive nitrogen balance is seen in
(A) Starvation
(B) Wasting diseases
(C) Growing age
(D) Intestinal malabsorption

37. Alanine can be synthesized from
(A) Glutamate and α-ketoglutarate
(B) Pyruvate and glutamate
(C) Pyruvate and α-ketoglutarate
(D) Asparate and α-ketoglutarate

38. All of the following are required for synthesis of alanine except
(A) Pyruvate
(B) α-ketoglutarate
(C) Glutamate
(D) Pyridoxal phosphate

 39. All of the following statements about aspartate are true except
(A) It is non-essential amino acid
(B) It is a dicarboxylic amino acid
(C) It can be synthesized from pyruvate and glutamate
(D) It can be converted into asparagine

 40. Glycine can be synthesized from
(A) Serine
(B) Choline
(C) Betaine
(D) All of these

 41. All of the following are required for synthesis of glutamine except
(A) Glutamate
(B) Ammonia
(C) Pyridoxal phosphate
(D) ATP

42. A coenzyme required for the synthesis of glycine from serine is
(A) ATP
(B) Pyridoxal phosphate
(C) Tetrahydrofolate
(D) NAD

 43. All of the following statements about proline are true except
(A) It is an imino acid
(B) It can be synthesized from glutamate
(C) It can be catabolised to glutamate
(D) Free proline can be hydroxylated to hydroxyproline

44. A protein rich in hydroxyproline is
(A) Prolamin
(B) Procollagen
(C) Collagen
(D) Proinsulin

45. All the following statement about hydroxyproline are true except
(A) There is no codon for hydroxyproline
(B) It is present in large amounts in collagen
(C) Free proline cannot be hydroxylated to hydroxyproline
(D) Hydroxylation of proline residues is catalysed by a dioxygenase

46. All of the following are required for hydroxylation of proline residues except
(A) Ascorbic acid
(B) Glutamate
(C) Ferrous ions
(D) Molecular oxygen

47. Cysteine can be synthesized from methionine and
(A) Serine
(B) Homoserine
(C) Homocysteine
(D) Threonine

48. Methionine is synthesized in human body from
(A) Cysteine and homoserine
(B) Homocysteine and serine
(C) Cysteine and serine
(D) None of these




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